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KMID : 0545119950050050280
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 5 p.280 ~ p.284
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Occurrence of OF4949II in the Fungal Mat formed by Surface Culture of Aspergillus niger F-580
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CHUN, HYO KON
CHUNG, MYUNG CHUL/KO, HACK RYONGKo, Hack Ryong/LEE, HO JAELee, Ho Jae/KHO, YUNG HEEKho, Yung Hee
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Abstract
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Aspergillus niger F-580, a potent producer of aminopeptidase M inhibitor, was isolated from the brown spots of plant leaves with a pathological trait. The inhibitory activity was found only in the fungal mat formed by surface culture of Aspergillus niger F-580, but not in the culture supernatant or cell pellet. The inhibitor was purified from the hot water extract of this fungal mat by using chromatographies on Diaion HP-20, DEAE-cellulose, Sephadex G-10 and YMC-ODS-AQ columns. The purified inhibitor was analyzed by UV, mass, and NMR spectroscopies, and identified as OF494911, which had been isolated as an aminopeptidase B inhibitor from Penicillium rugulosum OF4949
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